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Acquisition de données complémentaires aux dénombrements, avec les techniques de cytométrie en flux, fluorescence totale ou spectrale. Etat d’avancement et premiers résultats. Action Indice Composition. Livrable n° A III. Rapport final, 23 septembre 2014 ArchiMer
Artigas, Luis Felipe; Didry, Morgane; Barthelemy, Vanille; Bonato, Simon; Broutin, Mathias; Lizon, Fabrice; Lefebvre, Alain.
The European Union Water Framework Directive (WFD) refers to phytoplankton as one of the biological quality elements that should be regularly monitored and evaluated thanks to three ecological indices: biomass, abundance and composition. The first two have already been defined, but the third one has not yet been proposed in the English Channel-Atlantic zone. The construction of a composition indice must consider the diversity of all the phytoplankton. As the microscopic counts concern only the micro-phytoplankton, it is necessary to use additional information which concerns the nano- and pico-phytoplankton, of great importance when estimating the phytoplankton diversity. These components of the phytoplankton can be measured by different complementary...
Tipo: Text Palavras-chave: Manche Orientale; Groupes phytoplanctoniques; Cytométrie en Flux de type Scanning; Fluorimétrie spectrale; Analyses pigmentaires; Eastern English Channel; Phytoplankton groups; Scanning Flow Cytometry; Spectral fluorescence; Pigments analysis.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00361/47259/47224.pdf
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Utilisation conjointe de FlowCAM / ZooPhytoImage et de la cytométrie en flux. Premiers résultats et perspectives. Action 9. FlowCam ZooPhytoImage. Livrable n° 4. Rapport final, 23 septembre 2014 ArchiMer
Ali, Nour; Wacquet, Guillaume; Didry, Morgane; Hamad, Denis; Artigas, Luis Felipe; Grosjean, Philippe.
The goal of this study is to investigate about the possibility of coupling measurements made by image analysis from the FlowCAM with Zoo/PhytoImage with data obtained with a flux cytometer (pulse-shape-recording Scanning Flow Cytometry) on the same samples gathered in current monitoring networks in the eastern Channel and southern North Sea. In this preliminary study, we collected a series of samples off Boulogne-sur-Mer (SRN-REPHY monitoring system run by IFREMER) and along a transect in the Baie St-Jean (Wimereux-Slack) run by LOG. All these samples were digitized with a FlowCAM and measured with a scanning flow cytometer (CytoSense). The complete analysis with the FlowCAM and Zoo/PhytoImage is detailed in the present report. In order to get a better...
Tipo: Text Palavras-chave: Manche – Mer du Nord; Phytoplancton; Analyse d'image; Classification supervisée; Cytométrie en flux; Eastern English Channel and southern North Sea; Phytoplankton; Image analysis; Machine learning; Scanning Flow Cytometry.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00363/47442/47470.pdf
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